[GRAPHICS] This study aimed to clarify the initial 4-chlorophenol (4-CP) biodegradation pathway promoted by a two-component flavin-diffusible monooxygenase (TC-FDM) consisting of CphC-I and CphB contained in Arthrobacter chlorophenolicus A6 and the decomposition function of CphC-I. The TC-FDM genes were cloned from A. chlorophenolicus A6, and the corresponding enzymes were overexpressed. Since CphB was expressed in an insoluble form, Fre, a flavin reductase obtained from Escherichia coli, was used. These enzymes were purified using Ni2+-NTA resin. It was confirmed that TC-FDM catalyzes the oxidation of 4-CP and the sequential conversion of 4-CP to benzoquinone (BQN) -> hydroquinone (HQN) -> HQL. This indicated that CphC-I exhibits substrate specificity for 4-CP, BQN, and HQN. The activity of CphC-I for 4-CP was 63.22 U/mg-protein, and the Michaelis-Menten kinetic parameters were v(max) = 0.21 mM/min, K-M = 0.19 mM, and k(cat)/K-M = 0.04 mM(-1) min(-1). These results would be useful for the development of a novel biochemical treatment technology for 4-CP and phenolic hydrocarbons. (C) 2017 Elsevier Ltd. All rights reserved.