The present study investigates the synergistic effect of combined bacterial disintegration on mixed microalgal biomass for energy efficient biomethane generation. The rate of microalgal biomass lysis, enhanced biodegradability, and methane generation were used as indices to assess efficiency of the disintegration. A maximal dissolvable organics release and algal biomass lysis rate of about 1100, 950 and 800 mg/L and 26, 23 and 18% was achieved in PA + C (protease, amylase + cellulase secreting bacteria), C (cellulase alone) and PA (protease, amylase) microalgal disintegration. During anaerobic fermentation, a greater production of volatile fatty acids (1000 mg/L) was noted in PA + C bacterial disintegration of microalgal biomass. PA + C bacterial disintegration improve the amenability of microalgal biomass to biomethanation process with higher biodegradability of about 0.27 gCOD/g COD, respectively. The energy balance analysis of this combined bacterial disintegration of microalgal biomass provides surplus positive net energy (1.14 GJ/d) by compensating the input energy requirements. (C) 2017 Elsevier Ltd. All rights reserved.