Direct synthesis of dimethyl ether (DME) from syngas, was investigated over a CuO-ZnO-Al2O3 catalyst for methanol synthesis and a gamma-Al2O3 catalyst for a methanol dehydration. On the base of mathematical modeling, thermodynamic analysis was carried out in a wide range of pressures (10-100 bar) and temperatures (220-280 degrees C) for binary mixtures (H-2 + CO) with an H-2/CO = 1-6 M ratio. The influence of reaction conditions on the equilibrium content of components in the mixture was modelled. The effect of the loading method of catalysts of the methanol synthesis and its dehydration (layerwise loading, mixing) in the DME synthesis in a single reaction step on the CO conversion and the yield of DME was experimentally studied. It has been demonstrated that the layerwise combined loading with the intermediate mixed layer of CuO-ZnO-Al2O3 catalysts of the methanol synthesis and the gamma-Al2O3 catalyst for the DME synthesis (2: 1: 2, ratio parts, i.e. two parts of the catalyst of the methanol synthesis, one part of a mixture of the catalysts and two parts of the catalyst of the methanol dehydration) allow to obtain the higher yield of DME. The effect of operating conditions (pressure, temperature, ratio H-2/CO, time on stream, and catalyst stability) on the CO conversion and yield of DME was studied. The optimal conditions for loading variant 2: 1: 2 are: the pressure is 30 bar, the temperature is 280 degrees C and the initial ratio of H-2/CO is equal to 2. At that, the CO conversion remains practically constant during 180 h reaction run. (C) 2017 Published by Elsevier B.V.