There are several approaches to dyeing recipe formulation in the textile industry, because the accuracy of computer recipe prediction is not very high. There are several theories such as colorimetric and spectrophotometeric appraches on color reception. This work addressed the effect of the calibration method as the calculation of unit k/s and spectral overlapping of the components of the mixture on the accuracy of recipe prediction in dyeing nylon with acid dye. To this aim, the unit k/s was calculated using single and binary mixture samples. Two types unit k/s were used for the recipe prediction of ternary mixture dyed samples with low and high overlapping reflectance spectra. The obtained results indicated that the unit k/s of single component differed from the unit k/s of bicomponent mixture. The accuracy of recipe prediction using unit k/s of single component was less than that of the binary mixture. However, the performance of the recipe prediction of high level overlapping was found to be more than that of the low level overlapping mixture. Subsequently, the accuracy of color matching depended on the reflectance spectra similarity (spectral overlapping) of the components of the mixture.