Prussian blue (PB) films have been widely used for blood glucose monitoring. Here, we present the strategy to improve the performance and sensitivity of PB film for hydrogen peroxide (H2O2) monitoring by using modified gold electrode for hydrogen peroxide monitoring. The microstructure of the studied electrodes was characterized using scanning electron microscopy and atomic force microscopy. The electrochemical properties of experimental electrodes were obtained via cyclic voltammograms and chronoamperometry methods. The results show that the thickness of deposited PB film is increased with the deposition time. The PB-modified electrode exhibits the widest linear range and best operational stability after being electrochemically deposited for 240 s. The highest sensitivity for experimental electrodes is obtained on samples deposited for 40 s (341 mA cm(-2) M-1), indicating that a thinner PB film with certain critical thickness can accelerate the exchanging rate of K+ between PB lattice and the tested solution.