화학공학소재연구정보센터
Journal of Crystal Growth, Vol.476, 1-5, 2017
Time lapse microscopy of temperature control during self-assembly of 3D DNA crystals
DNA nanostructures are created by exploiting the high fidelity base-pairing interactions of doublestranded branched DNA molecules. These structures present a convenient medium for the selfassembly of macroscopic 3D crystals. In some self-assemblies in this system, crystals can be formed by lowering the temperature, and they can be dissolved by raising it. The ability to monitor the formation and melting of these crystals yields information that can be used to monitor crystal formation and growth. Here, we describe the development of an inexpensive tool that enables direct observation of the crystal growth process as a function of both time and temperature. Using the hanging-drop crystallization of the well-characterized 2-turn DNA tensegrity triangle motif for our model system, its response to temperature has been characterized visually. (C) 2017 Elsevier B.V. All rights reserved.