In this study, a simple, faster and eco-friendly method was used for the synthesis of piroxicam drug derived gold nanoparticles (PX-AuNPs) in aqueous solution. The electrochemical behavior of paracetamol (PAR) was investigated at a PX-AuNPs modified glassy carbon electrode (GCE) by using differential pulse voltrammetry (DPV) in Britton Robinson Buffer solution in strong acidic medium. It showed excellent electrocatalytic activity towards the anodic oxidation of PAR at the peak potential value of 0.66 V and considerable improvement in the peak currents as compared to that observed at the bare GCE. The advantages are related to the unique properties of modified nanosensor such as large surface area, catalytic behavior and hence increased electron transfer abilities compared to GC electrode. The calibration of peak current vs. concentration of PAR was linear in the range of 0.05-12 mu M with correlation coefficient R-2 = 0.996 and limit of detection (LOD) of 5 nM. We successfully applied this sensor for determination of PAR in drug samples with a mean recovery of 99.7%. The proposed sensor is simple, novel, rapid, reproducible and sensitive. It can be used as an alternative for chromatography and other voltammetric methods involving mercury electrode. The proposed method could be used for water analysis from pharmaceutical industrial effluents and so on. (C) 2017 The Electrochemical Society. All rights reserved.