A gold electrode (GE) modified with L-cysteine and gold nanoparticles (AuNPs) has been generated for computing the individual and simultaneous determination of nimesulide (NM) and acetaminophen (APAP). The electrochemical behavior of the pair of molecules has been inspected utilizing square wave voltammetry (SWV), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Studies indicated that the oxidation of APAP and NM is expedited at AuNPs/L-cysteine GE giving well defined peaks for APAP and NM at 0.596 V and 1.072 V respectively in Britton-Robinson (B-R) buffer (pH 2). The assay enabled simultaneous determination of these drugs in the linear working range of 3.0 x 10(-4) M-1.0 x 10(-5) M with detection limits of 4.06 x 10(-7) M and 6.49 x 10(-6) M for APAP and NM respectively. The high selectivity and sensitivity of the proposed assay were illustrated by its active application in the ascertainment of both APAP and NM in pharmaceutical preparations. (C) 2017 The Electrochemical Society. All rights reserved.