A post-cross-linked molecular imprinting (PC-MI) technique utilizing a functional polymer (FP) with interacting and post-cross-linking groups was developed to create molecularly imprinted polymeric (HIP) receptors. Molecular recognition cavities were formed in the cross-linked polymer matrix by a posteriori cross-linking of the FP with template molecules using photoirradiation. These cavities could be easily tuned to recognize the target molecules by changing the template using a common FP as a universal building block. Thus, precise chiral recognition cavities were successfully created using PC-MI and optimizing the molar ratio of the functional groups between the FP and the target molecules, which suppressed the nonspecific binding of the off target molecules. Furthermore, the morphology of the MIPs could be changed from bulk to particles. This study provides a facile and efficient synthetic route for MIPs with tailor-made properties. Thus, PC-MI can be utilized to create molecular recognition elements for purification, hygiene control, disease diagnosis, and sensors.