아실 글리콜 키토산이 첨가된 폴록사머 혼합 젤의 제조와 특성

김다은; 오혜민; 강선웅; 허강무; Da Eun Kim; Hye Min Oh; Sun-Woong Kang; Kang Moo Huh

Polymer(Korea), Vol.41, No.6, 1033-1040, November, 2017

DOI10.7317/pk.2017.41.6.1033 Export Citation

아실 글리콜 키토산이 첨가된 폴록사머 혼합 젤의 제조와 특성

Preparation and Characterization of Acyl Glycol Chitosan-Containing Poloxamer Gels

김다은^{1, 2}, 오혜민³, 강선웅^{3, 4, †}, 허강무^{3, †}

¹충남대학교 고분자공학과
² 안전성평가연구소
³안전성평가연구소
⁴과학기술연합대학원대학교 인체 및 환경 독성학과

Da Eun Kim^{1, 2}, Hye Min Oh¹, Sun-Woong Kang^{2, 3, †}, and Kang Moo Huh^{1, †}

¹Department of Polymer Science and Engineering, Chungnam National University, 99, Daehak-ro, Yuseong-gu, Daejeon 34134, Korea
²Predictive Model Research Center, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, Daejeon 34114, Korea
³Human and Environmental Toxicology Program, University of Science and Technology, 217, Gajeong-ro, Yuseong-gu, Daejeon 34113, Korea

E-mail:,

초록

폴록사머는 PEO-PPO-PEO 삼중블록 고분자로 수용액에서 온도 조절을 통해 가역적 젤을 형성할 수 있다는 장점으로 인하여 생체의료용 분야에 광범위하게 사용되고 있다. 하지만, 단일 폴록사머 젤은 수성 환경에서의 낮은 물리적 안정성으로 인해 생체재료로서의 적용에 한계가 있었다. 본 연구에서는 아실 글리콜 키토산의 혼합으로 폴록사머 젤의 물리화학적 특성을 개선하고자 하였다. 폴록사머와 아실 글리콜 키토산을 혼합하여 다양한 조성의 하이드로젤을 제조하였고, 온도 변화에 따른 졸-젤 상전이 특성과 물리적 안정성을 비교하였다. 아실 글리콜 키토산을 함유한 폴록사머 젤은 온도감응성과 물리화학적 특성이 향상됨과 더불어 낮은 세포독성을 보임으로써, 폴록사머의 생체재료서의 유용성을 보다 높일 수 있을 것으로 기대된다.

Poloxamers, PEO-PPO-PEO triblock copolymers, are well-known thermo-reversible sol-gel transition polymers that have great potential for use in biomedical applications, but have several drawbacks such as the weak mechanical properties and physical stability. In this study, we tried to modify and improve the physicochemical properties of poloxamer gels by preparation of acyl glycol chitosan-containing poloxamer gels. Various poloxamer gels with different compositions were prepared and characterized in terms of thermo-reversible sol-gel transition properties, mechanical properties, and physical stability. These hydrogel systems could demonstrate not only enhanced physicochemical and thermo-responsive properties but also low cytotoxicity, allowing poloxamer gels to extend their potential application area.

Keywords:poloxamer;acyl glycol chitosan;thermo-reversible sol-gel;physical stability

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[1] Ahn JS, Suh JM, Lee M, Jeong B, Polym. Int., 54, 842 (2005)

[2] Yu L, Ding J, Chem. Soc. Rev., 37, 1473 (2008)

[3] Moon HJ, Park MH, Joo MK, Jeong B, Chem. Soc. Rev., 41, 4860 (2012)

[4] Alexandridis P, Hatton TA, Colloids Surf. A: Physicochem. Eng. Asp., 96, 1 (1995)

[5] Dumortier G, Grossiord JL, Agnely F, Chaumeil JC, Pharm. Res., 23, 2709 (2006)

[6] Escobar-Chavez J, Lopez-Cervantes M, Naik A, Kalia Y, Quintanar-Guerrero D, Ganem-Quintanar A, J. Pharm. Pharm. Sci., 9, 339 (2006)

[7] Ruel-Gariepy E, Leroux JC, Eur. J. Pharm. Biopharm., 58, 409 (2004)

[8] Kabanov AV, Batrakova EV, Alakhov VY, J. Control. Release, 82, 189 (2002)

[9] Lee SH, Lee JE, Baek WY, Lim JO, J. Control. Release, 96, 1 (2004)

[10] Kempe S, Mader K, J. Control. Release, 161, 668 (2012)

[11] Akash MSH, Rehman K, J. Control. Release, 209, 120 (2015)

[12] Ur-Rehman T, Tavelin S, Grobner G, Int. J. Pharm., 409, 19 (2011)

[13] Lu C, Liu M, Fu H, Zhang W, Peng G, Zhang Y, Cao H, Luo L, Eur. J. Pharm. Sci., 77, 24 (2015)

[14] Abdi SIH, Choi JY, Lee JS, Lim HJ, Lee C, Kim J, Chung HY, Lim JO, Tissue Eng., 9, 1 (2012)

[15] Niu G, Du F, Song L, Zhang H, Yang J, Cao H, Zheng Y, Yang Z, Wang G, Yang H, Zhu S, J. Control. Release, 138, 49 (2009)

[16] Wu CJ, Gaharwar AK, Chan BK, Schmidt G, Macromolecules, 44(20), 8215 (2011)

[17] Ferreira SBDS, Moco TD, Borghi-Pangoni FB, Junqueira MV, Bruschi ML, J. Mech. Behav. Biomed. Mater., 55, 164 (2015)

[18] Kang YM, Kim LS, Lee SH, Son JS, Lee B, Kim JH, Kim MS, Polym. Sci. Technol., 20, 245 (2009)

[19] Lee Y, Chung HJ, Yeo S, Ahn CH, Lee H, Messersmith PB, Park TG, Soft Matter, 6, 977 (2010)

[20] Gratieri T, Gelfuso GM, Rocha EM, Sarmento VH, de Freitas O, Lopez RFV, Eur. J. Pharm. Biopharm., 75, 186 (2010)

[21] You HS, Cho MO, Cho IS, Li Z, Kim NH, Jang MK, Kang SW, Huh KM, Polym. Korea, 40, 1 (2016)

[22] Cho MO, Li Z, Shim HE, Cho IS, Nurunnabi M, Park H, Lee KY, Moon SH, Kim KS, Kang SW, Huh KM, NPG Asia Mater., 8, e309 (2016)

[23] Li Z, Cho S, Kwon IC, Janat-Amsbury MM, Huh KM, Carbohydr. Polym., 92, 2267 (2013)

[24] Edsman K, Carlfors J, Petersson R, Eur. J. Pharm. Sci., 6, 105 (1998)

[25] Kojarunchitt T, Hook S, Rizwan S, Rades T, Baldursdottir S, Int. J. Pharm., 408, 20 (2011)

[26] Bonacucina G, Spina M, Misici-Falzi M, Cespi M, Pucciarelli S, Angeletti M, Palmieri GF, Eur. J. Pharm. Sci., 32, 115 (2007)

[27] Amin MCIM, Ahmad N, Halib N, Ahmad I, Carbohydr. Polym., 88, 465 (2012)