The production of hydrogen from glycerol steam reforming has been studied in several reactors. In conventional reactors the catalyst is deactivated by coke: in fixed bed reactors plugging was observed if a low steam/glycerol ratio was employed, while in fluidized bed reactors it was possible to operate for a longer time-on-stream. The use of a two-zone fluidized bed reactor is studied in this work, as a method to counteract the problem of catalyst deactivation by coke. The glycerol reforming takes place in the upper part of this reactor while the catalyst is simultaneously regenerated in the lower part, where a stream of a regenerating gas is introduced. It has been found that CO2, O-2 or H2O can act as regenerating gas in a two-zone-fluidized bed reactor, allowing steady state operation at a water: glycerol molar ratio as low as 1.25. The effect of the operating conditions has been studied and the yield to the main products was compared with the calculated values assuming thermodynamic equilibrium.