This study was investigated on the mevinolin production from Penicillium citrinum Thom(KCTC 6990) according to the change of culture conditons, pH, temperature, carbon sources, nitrogen sources, surfactants, and glucose concentration. In pH changes its amount of production showed the order of 6.5, 7.0, 6.0. In temperature changes its amount of production showed the order of 24 ℃, 22 ℃, and 28 ℃. In carbon sources, its amount of production showed the order of glucose, glycerol, maltose, galctose. In nitrogen sources, its amount of production showed the order of peptone, soybean meal, NaNO3. In mineral kinds, its amount of production showed the order of K2HPO4, MgSO4, FeSO4. In kinds of surfactant, its amount of production showed the order of Tween 20, Tween 80 and in standard material, similarity to the result of polypropylene glycol 1000. In glucose concentration, its amount of production showed the most value in 120g/ℓ after 7 days, the case of continuous culture, the most value of 18.0mg/ℓ after 10 days, and the case of continuous feeding, 1.8 fold of that of intermittent feeding.