Senescence prompted after cutting and environmental exposure was evaluated in fresh cut papaya in two ripening stages and two tissue locations by confocal laser scanning microscopy and digital image analysis to stablishing tissue structural stability. Self-fluorescence images from two emission channels were analysed through multifractal parameters, lacunarity, and skeleton attributes. Skeletons features reflected tissue integrity by the number and length of branches, and amount of junctures as key elements in the microarchitecture of the cellular supportive structure, depending on pectin variation. Tissue stability could be described through the integrity of continuity lines given by the connected cell walls and middle lamella. The patterns of structural continuity lines showed properties of a multifractal set. Ripened and exposed tissues addressed lower singularity. Digital Image analysis allowed to determining stability status associated to tissue integrity and structural continuity by establishing singularities in heterogeneous tissue netting when describing senescence progression of fresh cut papaya. (C) 2017 Elsevier Ltd. All rights reserved.