Bio-mitigation of CO2 utilizing prokaryotes and simultaneous extraction of valuable biomolecule is fast gaining interest now-a-days. Present work discusses :the thermodynamic assessment of CO2 bio-mitigation capability of Pseudomonas aeruginosa SSL-4 isolated from halo alkalophilic habitat in the absence of light. The maximum specific growth (mu Max, h(-1)) of isolate was found to be 0.425 (+/- 0.0025) and 0.34 (+/- 0.0063) at 3% (w/v) salt concentration and 35 degrees C, respectively. The isolate was cultivated in the environment having initial CO2 (g) concentration of 17(0.8) % (v/v) using Fe[II] as an energy source (0, 50 and 100 ppm) for evaluating CO2 fixing ability of microorganisms. The maximum CO2 removal efficiency of 92.37 (2.46) % (v/v) was obtained at 100 ppm of Fe[II] concentration. The isolate has shown the maximum CO2 fixation rate (Rco(2))of 0.04 (0.003) and 0.06 (0.001) g/L/d at 50 and 100 ppm of Fe[II] concentration, respectively. FT-IR and GC -MS analysis of obtained leachate revealed the presence of fatty alcohols (C-12-C-28) and total product recovery (C-12-C-18) of 0.371 g per g of biomass. The thermodynamic assessment revealed the actual CO2 utilization efficiency of 41.16%. Thus, the isolated strain from extreme hyper saline environment has shown the potential for research dedicated to carbon capture and utilization. (C) 2017 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.