Restoration of chondrogenic properties of degenerative nucleus pulposus cells by repeated co-culture with adipose-derived stem cells

Jin-Su Kim; Daegi Kwon; Byung-Hyun Cha; Bo Kyung Moon; Younghyen Jeong; In-Bo Han; Hansoo Park; Soo-Hong Lee

Journal of Industrial and Engineering Chemistry, Vol.60, 185-191, April, 2018

DOI10.1016/j.jiec.2017.11.004 Export Citation

Restoration of chondrogenic properties of degenerative nucleus pulposus cells by repeated co-culture with adipose-derived stem cells

Jin-Su Kim^{1, 2}, Daegi Kwon¹, Byung-Hyun Cha¹, Bo Kyung Moon¹, Younghyen Jeong³, In-Bo Han⁴, Hansoo Park^{3, †}, and Soo-Hong Lee^{1, †}

¹Department of Biomedical Science, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea
²R&D Center, TJC LIFE Co. Ltd., 473-3, Bangbae-dong, Seocho-gu, Seoul, 06698, Republic of Korea
³School of Integrative Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul, 06974, Republic of Korea
⁴Department of Neurosurgery, Bundang Medical Center, CHA University, Seongnam-si, Gyeonggi-do, 13496, Republic of Korea

E-mail:,

We used porous membranes for co-culturing adipose-derived stem cells (ASCs) with nucleus pulposus cells (NPCs) to investigate whether repeated co-cultures restored the chondrogenic properties of degenerative NPCs (dNPCs). Human dNPCs were co-cultured with ASCs for 1 week and co-cultured again with new ASCs for 1 week. The expression levels of chondrogenic genes and GAGs were significantly higher in second co-cultured dNPCs than those in the first co-cultured dNPCs and in dNPCs cultured alone, respectively. After six weeks of in vivo transplantation, compared to first co-cultured dNPCs, second co-cultured dNPCs showed a significantly higher cartilage tissue formation.

Keywords:Repeated co-culture;Chondrogenic differentiation;Degenerated;Nucleus pulposus cell

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[1] Luoma K, Riihimaki H, Luukkonen R, Raininko R, Viikari-Juntura E, Lamminen A, Spine, 25, 487 (2000)

[2] O'Halloran DM, Pandit AS, Tissue Eng., 13, 1927 (2007)

[3] Acosta FL, Lotz J, Ames CP, Neurosurg. Focus, 19, E4 (2005)

[4] Sobajima S, Vadala G, Shimer A, Kim JS, Gilbertson LG, Kang JD, Spine J., 8, 888 (2008)

[5] Wang YT, Wu XT, Wang F, J. Orthop. Sci, 15, 707 (2016)

[6] Longo UG, Papapietro N, Petrillo S, Franceschetti E, Maffulli N, Denaro V, Stem Cells Int., 921053 (2012).

[7] Iyer NR, Wilems TS, Sakiyama-Elbert SE, Biotechnol. Bioeng., 114(2), 245 (2017)

[8] Richardson SM, Walker RV, Parker S, Rhodes NP, Hunt JA, Freemont AJ, Hoyland JA, Stem Cells, 24, 707 (2006)

[9] Mizuno H, Tobita M, Uysal AC, Stem Cells, 30, 804 (2012)

[10] Ding S, Kingshott P, Thissen H, Pera M, Wang PY, Biotechnol. Bioeng., 114(2), 260 (2017)

[11] Choi EH, Park KS, Han IB, Kang SW, Lee SH, Tissue Eng. Regen. Med., 6, 461 (2009)

[12] Fischer J, Dickhut A, Ewerbeck V, Richter W, Tissue Eng. Part A, 15, 705 (2009)

[13] Zhang FX, Hong Y, Liang WM, Ren TM, Jing SH, Lin JT, Biochem. Biophys. Res. Commun., 427(1), 86 (2012)

[14] Strassburg S, Richardson SM, Freemont AJ, Hoyland JA, Regen. Med., 5, 701 (2010)

[15] Yamamoto Y, Mochida J, Sakai D, Nakai T, Nishimura K, Kawada H, Hotta T, Spine, 29, 1508 (2004)

[16] Watanabe T, Sakai D, Yamamoto Y, Iwashina T, Serigano K, Tamura F, Mochida J, J. Orthop. Res., 28, 623 (2010)

[17] Meretoja VV, Dahlin RL, Kasper FK, Mikos AG, Biomaterials, 33, 6362 (2012)

[18] Choi EH, Park H, Park KS, Park KS, Kim BS, Han IB, Shin DA, Lee SH, Tissue Eng. Part A, 17, 2445 (2011)

[19] Gaetani P, Torre ML, Klinger M, Faustini M, Crovato F, Bucco M, Marazzi M, Chlapanidas T, Levi D, Tancioni F, Vigo D, Baena RRY, Tissue Eng. Part A, 14, 1415 (2008)

[20] Lu ZF, Doulabi BZ, Wuisman PI, Bank RA, Helder MN, Biochem. Biophys. Res. Commun., 359(4), 991 (2007)

[21] Lu ZF, Doulabi BZ, Wuisman PI, Bank RA, Helder MN, J. Cell. Mol. Med., 12, 2812 (2008)

[22] Ashraf S, Ahn J, Cha BH, Kim JH, Han I, Park H, Lee SH, J. Tissue Eng. Regen. Med., 11, 2503 (2016)

[23] Cha BH, Kim JS, Ahn JC, Kim HC, Kim BS, Han DK, Park SG, Lee SH, Biomaterials, 35, 2851 (2014)

[24] Donizetti-Oliveira C, Semedo P, Burgos-Silva M, Cenedeze MA, Malheiros DM, Reis MA, Pacheco-Silva A, Camara NO, Cell Transplant., 21, 1727 (2012)

[25] Pecanha R, Bagno LL, Ribeiro MB, Ferreira ABR, Moraes MO, Zapata-Sudo G, Kasai-Brunswick TH, Campos-de-Carvalho AC, Goldenberg RC, Werneck-de-Castro JPS, J. Bone Joint Surg. Am., 94, 609 (2012)

[26] Philips BJ, Marra KG, Rubin JP, Expert Opin. Biol. Ther., 12, 155 (2012)

[27] Hennig T, Lorenz H, Thiel A, Goetzke K, Dickhut A, Geiger F, Richter W, J. Cell. Physiol., 211, 682 (2007)

[28] van der Kraan PM, van den Berg WB, Osteoarthritis Cartilage, 20, 223 (2012)

[29] Cui JH, Chang HG, Kim YC, Tissue Eng. Regen. Med., 9, 92 (2012)

[30] Cha BH, Kim JH, Kang SW, Do HJ, Jang JW, Choi YR, Park H, Kim BS, Lee SH, Cell Transplant., 22, 1519 (2013)

[31] Wagner W, Bork S, Lepperdinger G, Joussen S, Ma N, Strunk D, Koch C, Aging, 2, 224 (2010)

[32] Blagosklonny MV, J. Cell. Physiol., 209, 592 (2006)