Kaempferol과 Nicotiflorin을 담지한 탄성 리포좀의 물리적 특성 및 In Vitro 피부 투과 연구

박소현; 신혁수; 윤믿음; 이상래; 송바름; 이난희; 박수남; So Hyun Park; Hyuk Soo Shin; Mid Eum Yun; Sang Lae Lee; Ba Reum Song; Nan Hee Lee; Soo Nam Park

Polymer(Korea), Vol.42, No.2, 330-338, March, 2018

DOI10.7317/pk.2018.42.2.330 Export Citation

Kaempferol과 Nicotiflorin을 담지한 탄성 리포좀의 물리적 특성 및 In Vitro 피부 투과 연구

Physical Characterization and In Vitro Skin Permeation of Elastic Liposomes Loaded with Kaempferol and Nicotiflorin

박소현, 신혁수, 윤믿음, 이상래, 송바름, 이난희^†, 박수남 ^†

서울과학기술대학교 정밀화학과 화장품종합기술연구소

So Hyun Park, Hyuk Soo Shin, Mid Eum Yun, Sang Lae Lee, Ba Reum Song, Nan Hee Lee^†, and Soo Nam Park^†

Department of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-Gu, Seoul 01811, Korea

E-mail:,

초록

본 연구에서는 kaempferol 및 nicotiflorin의 피부 전달 시스템으로 인지질(PC)과 계면활성제(cetearyl glucoside)로 구성된 탄성 리포좀을 제조하였으며, 그 물리적 특성 및 in vitro 피부 투과능을 평가하였다. Kaempferol을 담지한 리포좀의 입자 크기는 121.5~157.8 nm, 포집 효율은 67.5~81.2%, 가변형률은 2.8~17.6%로 나타났다. Nicotiflorin을 담지한 리포좀의 입자 크기는 125.1~143.1 nm, 포집 효율은 52.0~66.1%, 가변형률은 1.1~17.7%로 나타났다. In vitro 피부 투과 실험 결과, 계면활성제가 15%(w/w)인 ELK3 및 ELN3이 일반 리포좀인 CLK 및 CLN보다 더 높은 피부 투과능을 갖는 것을 확인하였다. 최적 조건으로 선정된 EL3은 kaempferol 및 nicotiflorin 피부 흡수 증진을 위한 유용한 전달체로 이용될 것으로 기대된다.

In this study, kaempferol and nicotiflorin loaded elastic liposomes (ELs) were investigated to enhance the transdermal delivery. The physical properties and in vitro permeation studies were performed using constructed ELs composed of egg phosphatidylcholine (PC) and edge activator (cetearyl glucoside). In kaempferol loaded liposomes, particle size ranged from 121.5 to 157.8 nm, deformability was recorded as 2.8~17.6%, and loading efficiency was from 67.5 to 81.2%. In nicotiflorin loaded liposomes, particle size ranged from 125.1 to 143.1 nm, deformability was recorded as 1.1~17.7%, and loading efficiency was from 52.0 to 66.1%. In vitro skin permeation studies demonstrated that elastic liposomes (ELK3 and ELN3) having 15%(w/w) cetearyl glucoside exhibited the higher skin permeability than CLK and CLN, the conventional liposome without cetearyl glucoside. The findings suggest that EL3 selected as the optimal formulation could be used as useful formulation for transdermal delivery of kaempferol and nicotiflorin.

Keywords:elastic liposome;kaempferol;nicotiflorin;transdermal delivery;skin permeation

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[2] Asare GA, Afriyie D, Ngala RA, Abutiate H, Doku D, Mahmood SA, Rahman H, Integr. Cancer Ther., 14, 65 (2015)

[3] Ishola IO, Awodele O, Olusayero AM, Ochieng CO, J. Med. Food, 17, 1375 (2014)

[4] Garcia-Mediavilla V, Crespo I, Collado PS, Esteller A, Sanchez-Campos S, Tunon MJ, Gonzalez-Gallego J, Eur. J. Pharmacol., 557, 221 (2007)

[5] Lee HB, Kim EK, Park SJ, Bang SG, Kim TG, Chung DW, J. Agric. Food Chem., 58, 4808 (2010)

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[7] Lunte SM, J. Chromatogr., 384, 371 (1987)

[8] Michaels AS, Chandrasekaran SK, Shaw JE, AlChE J., 21, 985 (1975)

[9] Otto DP, Villiers MM, Ther. Deliv., 5, 961 (2014)

[10] Montenegro L, Carbone C, Paolino D, Drago R, Stancampiano AH, Puglisi G, Int. J. Cosmet. Sci., 30, 57 (2008)

[11] Park SN, Lee MH, Kim SJ, Yu ER, Biochem. Biophys. Res. Commun., 435(3), 361 (2013)

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[13] Mura P, Maestrelli F, Gonzalez-Rodriguez ML Michelacci I, Ghelardini C, Rabasco AM, Eur. J. Pharm. Biopharm., 67, 86 (2007)

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[18] Aggarwal N, Goindi S, Int. J. Pharm., 437, 277 (2012)

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[20] Johnson W, Int. J. Toxicol., 20, 13 (2001)

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[22] Mosmann T, J. Immunol. Methods, 65, 55 (1983)

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