Blood supply is essential for the bone healing process to obtain successful osseointegration. a Calcitonin gene-related peptide (alpha CGRP) is osteoanabolic and is the most potent microvascular vasodilator currently known with validated angiogenic effect in vitro. We previously demonstrated that lentiviral alpha CGRP overexpression vector system could express the gene effectively to enhance titanium implant osseointegration. In this study, we investigated the effect of alpha CGRP on peri-implant angiogenesis during healing process in vivo. Based on investigation of blood vessel alteration in the peri-implant region of wild-type mice, we found a reduced angiogenesis alongside a decline in bone-implant contact percentage and bone mass in alpha CGRP mice. Overexpression of alpha CGRP could partly rescue the impairment Alterations were also detected in three-dimensional vascular structural parameters and expression of bone and vessel related genes. The results showed alpha CGRP increased vascular volume fraction and mean vessel size, as well as spatially relocated vessels approximate to the region of bone formation. And angiogenic and osteogenic genes were significantly upregulated in the transfection and alpha CGRP(+/+) group. These results suggested that aCGRP played a synergic role in angiogenesis and osseointegration, partly as a consequence of its vasodilative activity. (C) 2018 Published by Elsevier Inc.