Desmosomes are cell-cell adhesion junctions that anchor intermediate filaments. Loss of 14-3-3 gamma in HCT116 cells led to defects in desmosome assembly due to a decrease in the transport of Plakoglobin (PG) to the cell border thus disrupting desmosome formation. Desmosome formation in cells lacking 14-3-3 gamma was restored by artificially localizing PG to the cell border by fusing it to EGFP-f (PG-EGFP-f). These results suggest that a major role of 14-3-3 gamma in desmosome assembly is to transport PG to the cell border leading to the initiation of desmosome formation. (C) 2017 Elsevier Inc. All rights reserved.