Chemie Ingenieur Technik, Vol.90, No.3, 348-357, 2018
Continuous Countercurrent Extractive Biocatalysis in Aqueous Surfactant Two-Phase Systems
To evaluate the possibility of performing extractive biocatalysis in continuous mode, the hydrolysis of penicillin G in a micellar solution containing the nonionic surfactant Tergitol NP-7 was chosen as model system. While the product phenylacetic acid distributes into the micellar phase, 6-aminopenicillanic acid moves preferably into the aqueous phase. The yield in the continuous multi-step process was higher in comparison to equivalent batch systems. Overall, the results demonstrate the suitability of aqueous surfactant two-phase systems for continuous biocatalytic reactive extraction processes.
Keywords:Cloud point extraction;Continuous countercurrent extraction;Extractive biocatalysis;In situ product removal;Two-phase systems