To achieve green hydrolysis technology of hemicellulose through repeated using hydrolysate, the hydrolysis of hemicellulose in corncob was studied. The influence of repeated use of corncob hydrolysate on concentrations of D-xylose and L-arabinose was investigated. The loss rates of D-xylose in the prepared D-xylose solutions both with and without corncob, and repeated using corncob hydrolysate under identical acidity condition were discussed. The result shows that D-xylose concentration and L-arabinose concentration are all gradually increasing with the growing time of repeated use of corncob hydrolysate. After the fifth repetition, the concentrations of D-xylose and L-arabinose are 196.7 g.L-1 and 22.0 g.L-1, respectively. Substance inhibiting the degradation of D-xylose is generated during repeated use of corncob hydrolysate, and the production is further proved by the change of D-xylose concentration and the loss rate of D-xylose over heating time. (C) 2017 The Chemical Industry and Engineering Society of China, and Chemical Industry Press. All rights reserved.