Alternative fuels have been a major concern in today's era due to its promising characteristics in internal combustion engines. The computational analysis is a proven method by many researchers in determining combustion, performance and emission characteristics of an internal combustion engine, offering accurate results at par with experimental results. An attempt is being made by the author(s) in this paper for performing numerical study of nine (9) different alternative biofuels and pure diesel. Comparison has been carried out with the results of pure diesel in a single cylinder, naturally aspirated, water cooled, direct injection diesel engine with different engine load (20%, 40%, 60%, 80% and 100%), constant compression ratio (CR17.5), higher nozzle opening pressure (220 bar) and advance injection timing (23 degrees CA before TDC). The study was performed after validating results of two experimental data using the proposed tool, which have shown that the numerical results were in good agreement with the experimental results. The numerical results of the different biofuels have depicted the possibility of using the fuels as alternative fuel for internal combustion engine.