Significance of biodiesel is increasing forever because of increase in environmental pollution caused by fossil fuel and increasing of fossil fuel prices. Hence, the main goal of this study is to screen the effective process parameters for biodiesel production from chicken fat. Plackett-Burman (PB) design was used to screen the effective process variables among molar ratio, catalyst concentration (%), ultrasound power (W), mixing intensity (rpm), reaction time (min), temperature (degrees C), andmoisture level (%). The obtained biodiesel quality is compared with American Society for Testing andMaterials (ASTM) standard methods. This result confirms the effectiveness of PB design to screen the effective process parameters for biodiesel production.