Lignite from the Konin area (Poland) was used as a substrate for incubation experiments to evaluate its potential for simulation of biogenic methane production. Lignite was incubated with a bacterial inoculum enriched from lake sediments, mineral media, and various supplemental components for microbial life. Additives, such as acetate, methanol, glucose, nutrient broth, and yeast extract, can significantly increase methane production. At the same time, biodegradation of these additional carbon sources leads to overestimation of methane yield. In this paper, selected geochemical properties (total organic carbon content; stable isotopic composition of carbon delta C-13) were analyzed in order to evaluate changes in the organic matter of fermented lignite. Stable isotope analysis of carbon and hydrogen was applied in order to identify sources of methane and carbon dioxide formation. TOC decreased in range from 1.4 to 9.6% in lignite after fermentation. The delta C-13 value of lignite used in the experiments (-25.2 parts per thousand) decreased after incubation to values in range from -27.1 to -26.2 parts per thousand. Methane yield per g of TOC (lignite + organic carbon in nutrients) ranged from 0.47 to 2.60 mM/g. Glucose, acetate, and methanol significantly increased biogas production. Nutrient broth and yeast extract were not a source of organic carbon for methane formation, but their presence enhanced biogas production. Values of delta C-13(CH4) and delta H-2(CH4) across incubation conditions ranged from -70.2 to -24.2 parts per thousand and from -396.6 to -290.5 parts per thousand, respectively. Values of delta C-13(CO2) ranged from -55.2 to 45.0 parts per thousand. The high level of variation of delta C-13(CH4) and delta C-13(CO2) suggests mixing of gases from different carbon sources during incubation, but can also be caused by mixing of metabolic modes by the microbial community. Understanding the delta H-13(CH4) variation is even more difficult than the delta C-13(CH4) and delta C-13(CO2), since many substrates often contain exchangeable hydrogen (e.g. in water, lignite, and elements of nutrients). The combined values of delta C-13(CH4) and delta C-13(CO2) suggests that the dominant methanogenesis pathway in our experiments may be acetate fermentation. In biodegraded lignite, high relative concentrations of p-cresol (one of the most abundant in the sample) and n-(2-acetylphenyl)formamide were identified. These compounds are most probably lignin decomposition products, or, in the case of the latter, bacterial by-products or remnants. Organic compounds with low molecular weights, n-alkanes, and biomolecules including ferruginol, sugiol, and 6,7-dehydroferruginol, as well as amyrins and tocopherols, were preferentially degraded. The potential for methane production from lignite spiked with carbon-bearing additives is at least one magnitude lower than that from agricultural wastes. The lignite utilization as the single substrate for methanogenic fermentation is economically unprofitable. Mixing of lignite with the external substrate as biomass may be an alternative for consideration and future research.