The present study constructed a competitive recognition system using cell receptor screening for human papillomavirus (HPV) invasion by using the hybrid-assembly of polyoxometalates (POMs) and cationic peptides as a platform. The fine tuning both of the surface charge of POMs and peptide sequence were precisely performed to develop a luminescence switch of POMs, leading to the establishment of a ternary system to identify which types of glycosaminoglycans (GAGs) are potential cell receptors for HPV infection. In addition, the method was successfully applied to construct a hybrid-assembly with the recombined HPV 16 L1 pentamers from Escherichia coli and perform GAGs screening, which validated the system's potential for practical applications. In particular, the intrinsic mechanism for each competitive partner in the system was explained well by using isothermal titration calorimetry (ITC) and time resolved fluorescence spectra. The present method will be helpful to extend the protocol to other systems by using peptides and POMs with similar properties, and ultimately, we hope it will promote the development of anti-viral agents. (C) 2017 Elsevier Inc. All rights reserved.