Molybdenum oxide nanoparticles (MoOx, NPs) were successfully prepared by the pulsed laser ablation technique in water. Picosecond pulses allowed synthesizing chemically and morphologically stable MoOx colloidal nano-particles dispersed in water, which are used to fabricate modified screen printed carbon paste electrode (SPCE). The molybdenum oxide nanoparticles modified electrode (MoOx NPs/SPCE) shows enhanced electro-catalytic behavior for the detection of dopamine in Phosphate Buffered Saline (pH = 7) solution. Under the optimal conditions, the peak current of dopamine increased linearly with the concentration in the 0.1-600 mu M range, with a limit of detection (LOD) of 43 nM. The very easy MoOx NPs/SPCE fabrication, its high sensitivity, sub-micromolar detection limits and excellent selectivity towards main interferents, made them as a potential candidate for the detection of dopamine in pharmaceutical and clinical preparations.