The extraction of sapucaia (Lecythis pisonis) nut oil (SNO) using subcritical propane (SPE) and supercritical CO2 (with ethanol as co-solvent; scCO(2)) as solvent was investigated and compared with the conventional (Soxhlet) extraction. Extraction with scCO(2) was performed at 333 K and 20 MPa while the SPE extractions were carried out in different conditions to investigate the effects of temperature (293-333 K) and pressure (2-10 MPa) on the oil yield and the chemical compositions of the products. Results show that SPE allowed a fast extraction with a higher yield (46.22%) obtained at 333 K and 10 MPa, representing 93% efficiency compared to Soxhlet. Only temperature had significant (p < 0.05) effect on the extraction yield. SPE yielded the oils with highest values of polyunsaturated fatty acids (similar to 36%). Stability to oxidation ranges from 6.53 to 11.17 h. The major triacylglycerols present in SNO are OOO, SOO, POO, PLO, and POS.