In this work, Termamyl (R) 2X and Bioprep (R) 3000L were characterized and investigated for their antimicrobial activities by agar diffusion and Minimum Inhibitory Concentration (MIC). Bioprep (R) 3000L enzyme showed higher antimicrobial activity compared to the Termamyl (R) 2X against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Candida albicans, and was partially active for Pseudomonas aeruginosa, showing a high potential of application to antimicrobial textiles production replacing the synthetic compounds. In addition, Bioprep (R) 3000L enzyme was immobilized by covalent bond on the chemically modified cotton fabric surface via periodate reaction (20% immobilization yield) and its antimicrobial activity was investigated. (C) 2018 Elsevier B.V. All rights reserved.