Process Biochemistry, Vol.66, 205-211, 2018
Molecular characterization of peroxisome proliferator activated receptor gamma (PPAR gamma) in loach Misgurnus anguillicaudatus and its potential roles in fatty acid metabolism in vitro
Peroxisome proliferator activated receptor gamma (PPAR gamma) was firstly investigated in loach Misgurnus anguillicaudatus by RACE (rapid amplification of cDNA ends) and qPCR (real-time quantitative PCR). An in vitro cell culture experiment was then performed and showed that loach PPAR gamma positively affected expressions of four fatty acid metabolism related genes, namely fatty acid desaturase 2, lipoprotein lipase, elongase of very long chain fatty acids 5 and sterol regulatory element-binding protein-1c. At last, effects of C18:2n-6 (linoleic acid, LA) and C18:3n-3 (linolenic acid, LNA) on loach PPAR gamma expression were investigated. Loath fin cells cultured with 50 mu M and 100 mu M of LA or 100 mu M LNA exhibited significantly lower PPAR gamma transcriptions than those cultured with 0 mu M LA and LNA, showing that high-dose LA/LNA suppressed PPAR gamma expression in loath fin cells. Results of the two in vitro experiments suggested that PPAR gamma played an important role in fatty acid metabolism of the loath.
Keywords:Misgurnus anguillicaudatus;Peroxisome proliferator activated receptor gamma (PPAR gamma);Gene cloning;Spatio-temporal expressions;Fatty acid metabolism;In vitro