When is injected in a brine reservoir, brine or can be discharged into a permeable formation saturated with brine above the storage reservoir along a leakage pathway, if present. In most situations, the overlying formation can act as a single-phase aquifer with only brine leakage before leaks. This study examines the applicability of a developed inverse code for single-phase fluids to detect leakage pathway locations in view of the overlying formation using pressure anomalies induced by leaks. Before applying inverse analysis, forward modeling is performed using the TOUGH2 model to determine brine and leakage in a homogeneous conceptual model, and the simulated pressure profiles at monitoring wells are used as measurements in the inverse model. In the inverse code, an important consideration is that the vertical hydraulic conductivity and cross-sectional area of a leakage pathway that are inherent to a leakage term in the mass balance equation are integrated as a single parameter to estimate the leakage pathway locations. This method eliminates the impact of the uncertainty of the leakage pathway size on the accuracy of leakage pathway estimation. The inverse model examines the effect of the number of monitoring wells, monitoring periods and leakage into the overlying formation on the accuracy of leakage pathway estimation according to eleven application examples. The comparison between the results of the single-phase inverse code and iTOUGH2 code illustrates that the single-phase inverse model can be applicable to the leakage pathway estimation in a multiphase flow system.