Iron titanium catalysts with uniform mesopores were synthesized using cetyl trimethyl ammonium bromide (CTAB) as a template-directing agent, and applied to the selective catalytic reduction of NO with NH3. It was found that the catalyst exhibited high catalytic activity in a wide range of operating temperatures and good stability. The samples were characterized by means of N-2-physisorption, XRD, TEM, TPD, XPS and in situ DRIFTS technologies. Results showed that CTAB acted as both a "structural" and "chemical" promoter, which not only increased the specific surface area of the catalysts by optimizing the pore size, but also promoted the formation of a crystalline structure rather than an amorphous one, thereby leading to increased catalytic activity. Moreover, in situ DRIFTS revealed that Lewis acids played key roles in the SCR reaction, and the enhanced adsorption of bridged nitrate and coordinated NH3 on Lewis acids was an important reason for the excellent catalytic performance.