Implant- related infections are a consequence of microbial' adhesion to an implant surface followed by biofilm development on the implanted device. Chemical and biological functionalization of medical surfaces represents topical tactic of enhancing biocompatibility, together with the prevention of bacterial contamination and biofilm inhibition. For this purpose, we focused our work on laser surface modification of medical devices. We prepared nanoparticles of Fe3O4 loaded with Nigella sativa oil by a modified co-precipitation method in aqueous solution of NH4OH by oil-in-water emulsion. For further studies, we mixed them with poly(lactic-co-glycolic acid) spheres. The functionalized nanoparticles were deposited on glass and silicone surfaces by Matrix Assisted Pulsed Laser Evaporation technique. The influence of experimental factors, such as laser fluences was studied. The obtained thin coatings were analyzed by high resolution transmission electron microscopy, scanning electron microscopy and infrared microscopy. The most suitable coatings were selected and tested against a broad spectrum of pathogens found in the most implant rejection issues, such as S. aureus, E. coli. and C. albicans, at different time intervals and proved to limit microbial colonization and biofilm formation. In addition, biocompatibility studies using MG-63 cells adopting MTS assay confirmed the possible use of the obtained thin coatings to safely modify medical surfaces.