This study investigated a novel bioreactor configuration coupled with a side-stream dynamic membrane (DM) for Anammox enrichment as an alternative for conventional membrane. Bioreactor was fed with synthetic feed and seeded with a mix of anaerobic and aerobic sludge. In situ mechanical cleaning was employed for DM cleaning. DM development and performance was analysed over two polyamide-nylon meshes (200 and 52 mu m). Solid-liquid separation of 52 mu m mesh outperformed 200 mu m with an average effluent turbidity of 2.4 +/- 0.1 NTU. The system was operated at a maximum nitrogen loading rate of 696mg-NL-1 d(-1) and achieved a maximum nitrogen removal rate of 611.6 mg-NL(-1)d-(1). At steady state, the average ammonium, nitrite and total nitrogen removal efficiencies were 87 +/- 0.6%, 98.5 +/- 0.15% and 87.5 +/- 0.56% respectively. Digital realtime PCR Sequence analysis showed that Planctomycetales belonging to ascertained Anammox-specific genera progressively increased their presence in the reactor consistently with its nitrogen removal performance.