In this study, a novel biomass pretreatment process using three kinds of deep eutectic solvents (DESs) was developed to improve saccharification efficiency and lignin valorization. The major components of xylose residue including cellulose and lignin fractions were released, recovered and utilized. Pretreatment with betaine/ lactic acid system at 120 degrees C for 2 h was found to be the optimal conditions with prominently increased enzymatic digestibility (from 55.3% to 96.8%). The efficient conversion was mainly ascribed to the significant delignification efficiency of 81.6% after betaine/lactic acid pretreatment, which caused incompact structure and corrosive surface of treated xylose residue. The recoverable lignin had high purity, low molecular weight (630-2040 g/mol) and polydispersity (1.07-1.76). Based on the comprehensive analysis, the one-pot DESs system provides us a facile and effective approach for whole components valorization of lignocellulosic materials.