We developed a pilot-scale strengthened circulation anaerobic (SCA) reactor (with an effective volume of 27 m(3)) and applied to the treatment of industrial textile wastewater. The treatment performance and the working mechanism were studied systematically and the key operational parameters were identified. The results demonstrated that a stable and excellent chemical oxygen demand removal efficiency of 62.7% and a maximum chromaticity removal efficiency of 73.5% were obtained at an optimal reflux ratio of 4. Interestingly, the biodegradability was evidently improved after the SCA reactor treatment. The high throughput sequencing analysis indicated that the diversity of the bacteria or archaebacteria before the treatment was slightly higher than that after the treatment, which may be attributed to the production of certain toxic intermediates and/or characteristic pollutants during the treatment. Enzyme activity test and COD removal show that numerous microorganisms still maintained active in the anaerobic granular sludge even in a severe environment