This study investigated the greenhouse gas emission characteristics and microbial community dynamics with the variation of temperature during partial nitrification. Low temperature weakened nitrite accumulation, and partial nitrification would shift to complete nitrification easily at 15 degrees C. Based on CO2 equivalents (CO2-eq), partial nitrification process released 2.7 g of greenhouse gases per gMLSS per cycle, and N2O accounted for more than 98% of the total CO2-eq emission. The total CO2-eq emission amount at 35 degrees C was 45.6% and 153.4% higher than that at 25 degrees C and 15 degrees C, respectively. During partial nitrification, the microbial community diversity greatly declined compared with seed sludge. However, the diversity was enhanced at low temperature. The abundance of Betaproteobacteria at class level increased greatly during partial nitrification. Proteobacteria abundance declined while Nitrospira raised at low temperature. The nosZ community abundance was not affected by temperature, although N2O emission was varied with the operating temperature.