A biocatalytic system constructed by electrostatically immobilizing drug metabolizing human liver microsomes (HLM, negatively charged due to phospholipids) onto positively charged amine-functionalized magnetic nanoparticles (MNPamine, 100 nm hydrodynamic diameter) for biosensing of cytochrome P450 (CYP)-specific drug candidates and electrocatalyzing drug conversion into metabolites is reported. The MNP-adsorbed HLM biomaterial (denoted as MNPamine/HLM) was magnetically separated from the bulk suspension of HLM, washed twice in phosphate buffer (pH 7.0), and adsorbed onto polished edge-plane pyrolytic graphite (EPG) electrodes. Direct electrochemical properties and oxygen reduction currents were obtained by cyclic voltammetry (CV). Direct electron transfer kinetics from the microsomal redox proteins present in the adsorbed film of MNPamine/HLM on EPG surface was accomplished using square wave voltammetry due to its smaller background charging currents and improved faradaic peak intensity than CV. The MNPamine/HLM bioelectrodes exhibited a formal potential of -0.46 V vs Ag/AgCl reference (1M KCl), which was in agreement with the FAD/FMN cofactor containing CYP-reductase (CPR) as the electron receiver from the electrode. This conclusion was drawn based on the formal potentials of only CPR (similar to-0.47 V vs Ag/AgCl) containing bactosomal films adsorbed on EPG electrodes (bactosomes are bacterial membrane expressed enzymes). Whereas, bactosomal CYP 2C9 film showed a much more positive formal potential (-0.34 V vs Ag/AgCl). A heterogeneous electron transfer rate constant of 19 +/- 5 s(-1) was exhibited by the MNPamine/HLM film. Oxygen reduction currents and electrocatalytic diclofenac hydroxylation characteristics of the CYP enzymes present in the HLM film indicated the electron mediation by the CYP-reductase from the electrode to CYP-heme centers to initiate the catalytic cycle. Amperometric i-t curves with diclofenac drug concentration yielded the apparent Michaelis-Menten affinity constants (K-M(app)) of MNPamine/HLM and only HLM films as 302 +/- 33 and 365 +/- 36 mu M, respectively. This is the first electrocatalysis report of bioelectrodes featuring HLM-bound MNPamine for applications in drug activity assays, enhanced metabolite production, and sensitive biosensing of CYPspecific drugs and environmental pollutants. Eliminations of tedious isolation and purification of membrane-containing CYPs, and the need for expensive NADPH cofactor (as electrode donates the reducing equivalents in an inexpensive and sustainable manner) are added advantages. Our future work aims to gain quantitative metabolite estimations upon scalability of such bioelectrode designs useful for pharmacological and toxicological evaluations of new drugs in development. (C) 2018 Elsevier Ltd. All rights reserved.