We report here one-pot green synthetic method for preparing popcorn-like Au@Polyluminol nanoflowers by using the HAuCl4 to directly oxidize luminol at a specific controlled mass ratio of HAuCl4: luminol in hydrothermal conditions for the first time. The Au@Polyluminol nanoflowers were attached to the surface of a GCE electrode to form a highly solid-state electrochemiluminescent(ECL) sensor, which exhibited strong and stable ECL intensity, showed excellent ECL behavior. Further, the as-prepared Au@Polyluminol nanoflowers could also be used for the immobilization of uricase on an electrode surface to fabricate a sensitive solid-state Uric Acid ECL biosensor, which indicate that the Au@Polyluminol nanoflowers is a promising candidate for constructing various sensitive solid-state ECL sensors and promoting the application in electroanalytical and biochemical analysis. (C) 2018 Elsevier Ltd. All rights reserved.