Heat transfer from a pulsating laminar impingement slot jet on a flat surface was investigated numerically and experimentally. Inlet velocity was considered sinusoidal velocity and square wave velocity. Experimental studies were done only for the sinusoidal velocity state. An inverse heat conduction method, conjugated gradient method with adjoint equation, was used for the experimental estimation of the local heat transfer coefficient along the target surface. Effect of the square wave velocity of the laminar impingement slot jet was studied numerically. The results show pulsations in flow change flow patterns and the thermal boundary layer thickness because of the newly forming thermal boundary layer is extremely small each time the flow is resumed. Heat transfer rate in this state enhances due to pulsating inlet velocity in comparison with steady state. Heat transfer increases with increasing pulsation amplitude. Enhancement in mean heat transfer on the target plate for sinusoidal velocity is rather than square wave velocity.