Cost-effective non-noble metal catalysts are of key significance to the successful use of direct methanol fuel cells (DMFCs) for electricity generation. Herein, cuprous oxide nano particles (Cu2O NPs) supported graphene oxide (GO), polypyrrole (PPy) and polypyrrole-graphene oxide (PPy-GO) matrices were prepared using borohydride reduction method. The prepared catalysts were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-Vis spectra, Zeta potential and transmission electron microscopy (TEM). The elemental analysis of the composites was done by energy dispersive X-ray spectroscopy (EDX). Cu2O NPs were homogeneously dispersed and strongly anchored on the PPy grafted GO matrix and this was examined through morphological analysis. The Cu2O/PPy-GO (80:10:10) NPs exhibited noticeable improvement in electrochemical performance in comparison to pure graphene oxide (GO) and pure PPy supported Cu2O NPs catalyst and revealed the peak current density of 300 mu A cm(-2) at +0.68 V. The Cu2O/PPy-GO system demonstrated higher current density and also exhibited greater stability in comparison to the commercial Pt-Ru/C catalyst as characterized by chronoamperometry (CA) analysis. This prospective nano-catalyst showed higher I-F/I-B ratio (26%, 8.6% and 19%) compared to the corresponding catalyst systems of Cu2O/GO, Cu2O/PPy and Pt-Ru/C. In direct methanol fuel cell (DMFC), the efficiency of Cu2O/PPy-GO nano-catalyst system as an anode catalyst for methanol oxidation reaction (MOR) was investigated and the result revealed a maximum current density of 155 mA cm(-2) at +0.2 V and power density of 31 mW cm(-2). Hence, Cu2O/PPy-GO NPs are a cost-effective alternative for Pt-Ru/C system to execute practical application in DMFC. (C) 2017 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.