Although polymeric membranes find important role in water and waste water treatment in recent years, their fouling is still an important problem. Application of hydrophilic nanoparticles (NPs) is one of the proposed methods for reducing fouling of membranes but their dispersion and stability in hydrophobic polymer matrix is challenging. In this study Janus functionalization of the NPs was introduced as a promising technique toward achieving this goal. Polysulfone (PSf) membranes containing various concentrations of graphene oxide (GO) nanosheets and Janus graphene oxide ((anus GO) nanosheets (as additives) were fabricated via phase inversion. The synthesized nanosheets were characterized by field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy and dynamic light scattering (DLS). The prepared membranes also were then characterized by scanning electron microscopy (SEM), contact angle (CA). water uptake, porosity, mean pore size and casting solution viscosity. The membrane performance was also tested by determining pure water flux (PWF), bovine serum albumin (BSA) separation, flux reduction by fouling and flux recovery. CA reduced from 85 to 68 and PWF increased from 23.15 L/m(2) h to 230.61 L/m(2) h for PSF and Janus GO nanosheets containing membrane, respectively. Also investigation of antifouling performance of membranes revealed that membrane with the 1 wt.% of Janus GO nanosheets had higher water flux recovery ratio (FRR) and lower irreversible fouling (R-ir) of 84% and 16%, respectively. These improvements were attributed to the better dispersion and stability of Janus GO nanosheets in the prepared mixed matrix membranes. (C) 2018 Elsevier Inc. All rights reserved.