The present study was aimed to investigate the direct electrooxidation of bovine serum albumin (BSA) without stabilizer and adsorbent to design antifouling screen printed carbon interface. Herein, a simple strategy revealed good immobilization support for biological molecules, better conducting transducer interface, and above all improved antifouling properties which are typically contradictory in one material. BSA was electro grafted onto the carbon surface of screen printed electrode in presence of NBu4BF4. Furthermore, to inhibit non-specific adsorption diethanolamine (DEA) was used as a blocking agent followed by adsorption studies. The comprehensive study of various BSA protein concentrations, pH buffer solutions and interaction of different analyte molecules such as DNA and toxin molecules was conducted for the adopted procedure as model analytes. The BSA grafting protocol was also validated by comparing the analyte adsorptions with simply drop casted modifications. The obtained results not only demonstrated the antifouling characteristics of the transducer surface but also proved the obtained transducer surface as a conductive immobilization support. Most of the medical devices face complications and failures due to nonspecific biofouling of microorganisms, cells and proteins. Towards imparting these devices with nonfouling capabilities, this stabilizer free SBA grafting may result a highly promising bio-surface for biomedical and engineering applications.