Influence of roller and ball mill grinding technology applied for sucrose particles in a lipophilic suspension was investigated considering both particle properties and suspension's flow behavior. Especially particle surfaces after grinding were analyzed using a novel approach by combining atomic force microscopy (AFM) and AFM-local thermal analysis (AFM-LTA). This technique is able to characterize local distributions of different surface states on sucrose particles in nano- and microscale by determining local softening temperatures. For the first time, it was possible to demonstrate on molecular level that applied grinding technologies resulted in different surface characteristics with respect to adhesion forces and state of sucrose using AFM-LTA. Differences in flow behavior despite same particle size distributions and solid contents were traced back to the distribution of crystalline and amorphous areas on sucrose particle surfaces. (C) 2018 Elsevier Ltd. All rights reserved.