Poly(N-methylpyrrole) nanotubes were facilely fabricated by in situ chemical polymerization via a reactive template of MnO2 and were developed as electrode materials for the detection of dopamine. The microstructure, morphology, and chemical composition of the as-prepared poly(N-methylpyrrole) nanotubes have been investigated by X-ray powder diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Furthermore, a glassy carbon electrode (GCE) was modified with poly(N-methylpyrrole) nanotubes and showed sensitive catalytic activity for electrochemical detection of low concentration of dopamine even in the presence of and ascorbic acid.