화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.26, No.8, 755-762, August, 2018
DOI10.1007/s13233-018-6095-z  Export Citation
Laccase Immobilization onto Magnetic β-Cyclodextrin-Modified Chitosan: Improved Enzyme Stability and Efficient Performance for Phenolic Compounds Elimination
Roghayeh Tarasi1, Masoumeh Alipour2, Lena Gorgannezhad3, Somaye Imanparast4, Aliakbar Yousefi-Ahmadipour4, Ali Ramezani1, Mohammad Reza Ganjali5, 6, Abbas Shafiee7, Mohammad Ali Faramarzi4, †, and Mehdi Khoobi2, 8, †
  • 1Department of Chemistry, University of Zanjan, Zanjan, Iran
  • 2The Institute of Pharmaceutical Sciences, Tehran University of Medical Sciences, Tehran 1417614411, Iran
  • 3School of Natural Sciences, Griffith University, Nathan Campus, QLD 4111, Australia
  • 4Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155–6451, Tehran 1417614411, Iran
  • 5Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran
  • 6Biosensor Research Center, Endocrinology & Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  • 7The Institute of Pharmaceutical Sciences, Tehran University of Medical Sciences, Tehran 1417614411, Iran, Korea
  • 8Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155–6451, Tehran 1417614411, Iran
E-mail:,
Three types of improved Fe3O4 magnetic nanoparticles (MNPs), including poly(amidoisophthalicacid) coated magnetite nanoparticles (Fe@PA), cyclodextrin (CD) anchored Fe@PA (Fe@PA-CD), and chitosan (Cs) coated Fe@PA-CD (Fe@PACD-Cs) were successfully developed and characterized. Laccase immobilization onto MNPs was carried out via physical adsorption. The maximal and minimal loading capacity were obtained for Fe@PA and Fe@PA-CD-Cs, respectively. Fe@PA-CDCs-laccase exhibited around 100% of the maximum activity at pH 4 and maintained 70% of its initial activity within the temperature range of 15?55 °C; and Cs coated nanoparticles were more efficient than non-coated. Fe@PA-CD-Cs-laccase maintained 70% of its initial activity up to 12 d from the first day of storage at 25 °C whereas the free laccase, Fe@PA-laccase, and Fe@PA-CD-laccase kept 10%, 28%, and 33% of initial activity, respectively. Furthermore, bio-removal of phenolic compounds was performed by the free and immobilized enzyme. Fe@PA-CD-Cs-laccase showed maximal removal with 96.4% and 85.5% for phenol and bisphenol A, respectively. It seems that Fe@PA-CD-Cs could be an appropriate support for immobilization of other enzymes in various industrial application especially bioremoval of phenolic compounds.
Keywords:magnetic nanoparticles;chitosan;laccase;immobilization;enzyme stability
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