Preparation of CS/PVA Nanofibrous Membrane with Tunable Mechanical Properties for Tympanic Member Repair

Mengjia Huang; Jie Li; Jia Chen; Mi Zhou; Jianguo He

Macromolecular Research, Vol.26, No.10, 892-899, October, 2018

DOI10.1007/s13233-018-6127-8 Export Citation

Preparation of CS/PVA Nanofibrous Membrane with Tunable Mechanical Properties for Tympanic Member Repair

Mengjia Huang, Jie Li, Jia Chen¹, Mi Zhou^†, and Jianguo He^{1, †}

College of Materials Science and Engineering, Zhejiang University of Technology, Hangzhou, P. R. China
¹Departments of Otolaryngology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, P. R. China

E-mail:,

The wound healing for tympanic membrane (TM) perforations is one of the most common clinic treatment. New designs of biomaterials, and lately tissue-engineered composites and grafts, have been applied for the management of TM perforation. In this work, a series of chitosan (CS)/polyvinyl alcohol (PVA) nanofibrous films have been fabricated as a kind of patch for repairing TM perforation. The scaffolds were crosslinked by glutaraldehyde solution to prevent them from being dissolved in the culture medium. The morphology of nanofibers from different precursors has been observed by using scanning electron microscopy (SEM). And the crosslinked films have been characterized by SEM and Fourier transform infrared spectroscopy for confirming its morphology and chemical structure. We found the films with crosslinking showed stronger tensile strength and modulus compared to the uncrosslinked one. And the mechanical properties could be tuned by the crosslinking time. In addition, after crosslinked, the films showed good thermostability and water-resistance, characterized by thermo gravimetric analyzer and contact angle meter. The cell viability studies with human umbilical vein endothelial cells, fibroblasts and mesenchymal stem cells demonstrated that the nanofibrous scaffolds formed by electrospinning process had no side effects for cell proliferation. However, a better way of crosslinking CS/PVA films needs to be further studied.

Keywords:chitosan;nanofibrous membrane;tunable mechanical properties;tympanic membrane

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[2] Evans ND, Oreffo RO, Healy E, Thurner PJ, Man YH, J. Mech. Behav. Biomed., 28, 397 (2013)

[3] Rho KS, Jeong L, Lee G, Seo BM, Park YJ, Hong SD, Roh S, Cho JJ, Park WH, Min BM, Biomaterials, 27, 1452 (2006)

[4] Shen Y, Redmond SL, Teh BM, Yan S, Wang Y, Zhou L, Budgeon CA, Eikelboom RH, Atlas MD, Dilley RJ, Zheng MH, Marano RJ, Tissue. Eng. Part A, 19, 657 (2013)

[5] Kim SW, Kim J, Seonwoo H, Jang KJ, Kim YJ, Lim HJ, Lim KT, Tian C, Chung JH, Choung YH, Sci. Rep., 5, 11542 (2015)

[6] Zahnert T, Huttenbrink KD, Bornitz M, Am. J. Otol., 21, 322 (2000)

[7] Singh GB, Sharma A, Singh N, J. Otolaryngol., 35, 408 (2006)

[8] Angeli SI, Kulak JL, Guzman J, Laryngoscope, 116, 1594 (2006)

[9] Camnitz PS, Bost WS, Otolaryngol. Head Neck Surg., 93, 220 (1985)

[10] Carr SD, Strachan DR, Raine CH, J. Laryngol. Otol., 129, 23 (2015)

[11] Suzuki H, Koizumi H, Kitamura T, Tabata T, Kise Y, Hashida K, J. UOEH, 38, 237 (2016)

[12] Levin B, Rajkhowa R, Redmond SL, Atlas MD, Expert Rev. Med. Devices, 6, 653 (2009)

[13] Teh BM, Marano RJ, Shen Y, Friedland PL, Dilley RJ, Atlas MD, Tissue Eng. Part B: Rev., 19, 116 (2013)

[14] Farhadi M, Mirzadeh H, Solouk A, Asqhari A, Jalessi M, Ghanbari H, Yazdanifard P, J. Biomed. Mater. Res. A, 549 (2012)

[15] Sundback CA, McFadden J, Hart A, Kulig KM, Wieland AM, Pereira MJ, Pomerantseva I, Hartnick CJ, Masiakos PT, J. Biomed. Mater. Res. B, 100, 1943 (2012)

[16] Ghassemifar R, Redmond S, Zainuddin, Chirila TV, J. Biomater. Appl., 24, 591 (2010)

[17] Chauvin K, Bratton C, Parkins C, Otolaryngol. Head Neck Surg., 121, 43 (1999)

[18] Weber DE, Semaan MT, Wasman JK, Beane R, Bonassar LJ, Megerian CA, Laryngoscope, 116, 700 (2006)

[19] Kim JH, Bae JH, Lim KT, Choung PH, Park JS, Choi SJ, Im AL, Lee ET, Choung YH, Chung JH, J. Biomed. Mater. Res. Part A, 90A, 446 (2009)

[20] Au HT, Pham LN, Thi Vu TH, Park JS, Macromol. Res., 20(1), 51 (2012)

[21] Bhattarai N, Edmondson D, Veiseh O, Filmsen FA, Zhang MN, Biomaterials, 26, 6176 (2005)

[22] Sienknecht UJ, Hearing Res., 301, 19 (2013)

[23] Ko JH, Yin H, An J, Chung DJ, Kim JH, Lee SB, Pyun DG, Macromol. Res., 18(2), 137 (2010)

[24] Charernsriwilaiwat N, Rojanarata T, Ngawhirunpat T, Opanasopit P, Int. Wound J., 11, 215 (2014)

[25] Ohkawa K, Cha D, Kim H, Nishida A, Yamamoto H, Macromol. Rapid Commun., 25, 1600 (2010)

[26] Mohamad SH, Khan I, Hussain SS, Otol. Neurotol., 33, 699 (2012)

[27] Singh BJ, Sengupta A, Das S, Ghosh D, Basak B, Indian J. Otolaryngol. Head Neck Surg., 61, 131 (2009)

[28] Min BM, Lee SW, Lim JN, You Y, Lee TS, Kang PH, Park WH, Polymer, 45(21), 7137 (2004)

[29] Geng X, Kwon OH, Jang J, Biomaterials, 26, 5427 (2005)

[30] Vondran JL, Sun W, Schauer CL, J. Appl. Polym. Sci., 109(2), 968 (2008)

[31] Li Y, Qiu T, Xu X, Eur. Polym. J., 49, 1487 (2013)

[32] Shin US, Park JS, Oh JS, Lee EJ, Macromol. Res., 25(11), 1057 (2017)