화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.67, 244-254, November, 2018
DOI10.1016/j.jiec.2018.06.035  Export Citation
Polydopamine-mediated surface modifications of poly L-lactic acid with hydroxyapatite, heparin and bone morphogenetic protein-2 and their effects on osseointegration
Young Jin Yun, Han-Jun Kim1, Deok-Won Lee2, Sewook Um3, and Heung Jae Chun†
  • Institute of Cell & Tissue Engineering, Department of Biomedical Sciences, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea
  • 1Department of Clinical Pathology, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea
  • 2Department of Oral and Maxillofacial Surgery, Kyung Hee University Dental Hospital at Gangdong, Kyung Hee University, Seoul 05278, Republic of Korea
  • 3Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea
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Surface modified poly L-lactic acid (PLLA) samples with hydroxyapatite (HA), heparin and bone morphogenetic protein-2 (BMP-2) mediated by polydopamine (pDA) coating (PLLA/pDA/HA/Hep/BMP-2) were prepared, and their effects on the enhancements of bone formation and osseointegration were evaluated in vitro and in vivo as compared to PLLA, PLLA/pDA/HA, and PLLA/pDA/Hep/BMP-2. The changes in surface chemical compositions, morphologies and wettabilities were observed by Xray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM) and water contact angle measurements. Pre-coating of HA particles with pDA provided uniform and homogeneous anchoring of particles to PLLA surface. In addition, the strong ionic interaction between heparin and pDA led PLLA surface readily heparinized for loading of BMP-2. In vitro experiments revealed that the levels of alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin (OCN) gene expression were higher in MG-63 human osteosarcoma cell lines grown on PLLA/pDA/HA/Hep/ BMP-2 than on control PLLA, PLLA/pDA/HA, and PLLA/pDA/Hep/BMP-2. In vivo studies using micro- computed tomography (micro-CT) also showed that PLLA/pDA/HA/Hep/BMP-2 screw exhibited greatest value of bone volume (BV) and bone volume/tissue volume (BV/TV) among samples. Histological evaluations with H&E and Von Kossa staining demonstrated that a combination of HA and BMP 2 contributed to the strong osseointegration.
Keywords:Polydopamine;Poly L-lactic acid;Hydroxyapatite;Bone morphogenetic protein-2;Bone formation
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