Visible light-cured glycol chitosan hydrogel dressing containing endothelial growth factor and basic fibroblast growth factor accelerates wound healing in vivo

Youngbum Yoo; Hoon Hyun; Sun-Jung Yoon; So Yeon Kim; Deok-Won Lee; Sewook Um; Sung Ok Hong; Dae Hyeok Yang

Journal of Industrial and Engineering Chemistry, Vol.67, 365-372, November, 2018

DOI10.1016/j.jiec.2018.07.009 Export Citation

Visible light-cured glycol chitosan hydrogel dressing containing endothelial growth factor and basic fibroblast growth factor accelerates wound healing in vivo

Youngbum Yoo, Hoon Hyun¹, Sun-Jung Yoon², So Yeon Kim³, Deok-Won Lee⁴, Sewook Um⁵, Sung Ok Hong⁶, and Dae Hyeok Yang^{7, †}

Department of Surgery, School of Medicine, The Konkuk University, Seoul 05030, Republic of Korea
¹Department of Biomedical Sciences, Chonnam National University Medical School, Gwangju 61469, Republic of Korea
²Department of Orthopedic Surgery, Research Institute of Clinical Medicine of Chonbuk National University, Biomedical Research Institute of Chonbuk National University Hospital, Jeonju 54907, Republic of Korea
³Department of Dental Hygiene, College of Health Sciences, Cheongju University, Cheongju 28503, Republic of Korea
⁴Department of Oral & Maxillofacial Surgery, Kyung Hee University Dental Hospital at Gangdong, Kyung Hee University, Seoul 153-782, Republic of Korea
⁵Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea
⁶Department of Dentistry, Catholic Kwandong University, School of Medicine, International St. Mary’s Hospital, Incheon 22711, Republic of Korea
⁷Institute of Cell and Tissue Engineering, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea

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Wounds that heal with excessive scar formation result in poor functional and aesthetic outcomes. To address this, in our study, visible light cured glycol chitosan (GCH) hydrogels containing endothelial growth factor (EGF) and basic fibroblast growth factor (bFGF) were prepared (GCH-EGF, GCH-FGF and GCH-EGF/FGF) and evaluated their efficacies on the improvement of wound healing in vivo. In vitro release test showed that the growth factors were released in a sustained manner along with initial burst for 24 h. In vitro cell proliferation assay of L-929 mouse fibroblast cell line resulted in the superior ability of GCH-EGF/FGF on the rate. In vivo results demonstrated that the growth factor loaded GCHs further enhanced wound healing compared with GCH. In particular, GCH-EGF/EFG showed the most remarkable wound healing effect among the samples.

Keywords:Endothelial growth factor (EGF);Basic fibroblast growth factor (bFGF);Glycol chitosan (GCH);Visible light curing;Wound healing

[References]

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[1] Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomic-Canic M, Wound Rep. Reg., 16, 585 (2008)

[2] Lynch SE, Colvin RB, Antoniades HN, J. Clin. Invest., 84, 640 (1989)

[3] Yang DH, Seo DI, Lee DW, Bhang SH, Park KS, Jang G, Kim CH, Chun HJ, J. Ind. Eng. Chem., 53, 360 (2017)

[4] Hardwicke J, Schmaljohann D, Boyce D, Thomas D, Surgeon, 6, 172 (2008)

[5] Akita S, Akino K, Hirano A, Adv. Wound Care, 2, 44 (2013)

[6] Park JW, Hwang SR, Yoon IS, Molecules, 22, 1259 (2017)

[7] Liu H, Wang C, Li C, Qin Y, Wang Z, Yang F, Li Z, Wang J, RSC Adv., 8, 533 (2018)

[8] Yoon SJ, Hyun H, Lee DW, Yang DH, Molecules, 22, 1513 (2017)

[9] Di Z, Yoshihiro I, Sci. China Chem., 57, 510 (2014)

[10] Darby IA, Laverdet B, Bonte F, Desmouliere A, Clin. Cosmet. Investig. Dermatol., 7, 301 (2014)

[11] Sood A, Granick MS, Tomaselli NL, Adv. Wound Care, 3, 511 (2014)

[12] Lee K, Silva EA, Mooney DJ, J. Royal Soc. Interface, 8, 153 (2011)

[13] Cohen S, Dev. Biol., 12, 394 (1965)

[14] Brown GL, Curtsinger LJ, White M, Mitchell RO, Pietsch J, Nordquist R, von Fraunhofer A, Schultz GS, Ann. Surg., 208, 788 (1988)

[15] Schultz G, Rotatori DS, Clark W, J. Cell. Biochem., 45, 346 (1991)

[16] Brown GL, Nanney LB, Griffen J, Cramer AB, Cramer JM, Yancey JM, Curtsinger LJ, Holtzin L, Schultz GS, Jurkiewicz MJ, Lynch JB, N. Engl. J. Med., 321, 76 (1989)

[17] Shi HX, Lin C, Lin BB, Wang ZG, Zhang HY, Wu FZ, Cheng Y, Xiang LJ, Guo DJ, Luo X, Zhang GY, Fu XB, Bellusci S, Li XK, Xiao J, PLOS One, 8, e59966 (2013)

[18] Akita S, Akino K, Hrano A, Adv. Wound Care, 2, 44 (2013)

[19] Batchelor RR, Kwandou G, Spicer PT, Stenzel MH, Polym. Chem., 8, 980 (2017)

[20] Bettini R, Colombo P, Massimo G, Catellani PL, Vitali T, Eur. J. Pharm. Sci., 2, 213 (1994)

[21] Song A, Rane AA, Christman KL, Acta Biomater., 8, 41 (2012)

[22] Koh TJ, DiPietro LA, Expert Rev. Mol. Med., 13, e23 (2011)

[23] Troidl C, Mollmann H, Nef H, Masseli F, Voss S, Szardien S, Willmer M, Rolf A, Rixe J, Troidl K, Kostin S, Hamm C, Elsasser A, J. Cell. Mol. Med., 13, 3485 (2009)

[24] Bodnar RJ, Adv. Wound Care, 2, 24 (2013)

[25] Eming SA, Martin P, Tomic-Canic M, Sci. Transl. Med., 6, 265sr6 (2014)