화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.67, 461-468, November, 2018
DOI10.1016/j.jiec.2018.07.020  Export Citation
Utility of solid phase extraction for colorimetric determination of lead in waters, vegetables, biological and soil samples
Zakia Al-Mallah, and Alaa S. Amin1, †
  • Chemistry Department, Faculty of Applied Science, Umm-Quarh University, Saudi Arabia
  • 1Chemistry Department, Faculty of Science, Benha University, Benha, Egypt
E-mail:
A highly sensitive, selective and rapid method for the determination of lead based on the reaction of lead (II) with 5-(4'-chlorophenylazo)-6-hydroxypyrimidine-2,4-dione (CPAHPD) and the solid phase extraction of the Pb(II).CPAHPD complex with Amberlite XAD-2000 was developed, in the presence of pH 5.6 buffer solution and Triton X-114 medium. CPAHPD reacts with lead to form a violet complex with a molar ratio of 2:1 (CPAHPD to lead). This complex was enriched by the solid phase extraction with Amberlite XAD-2000. An enrichment factor of 500 was obtained by elution of the complex from the resin with a minimal amount of isopentyl alcohol (0.2 mL). In isopentyl alcohol medium, the molar absorptivity of the complex is 1.13 X 106 L mol-1cm-1 at 647 nm. Beer’s law is obeyed in the range of 5.0-160 ng mL-1 in the measured solution. The relative standard deviation for 10 replicate samples of 50 ng mL-1 level is 1.26%. The detection and quantification limits reaches 1.5 and 4.7 ng mL-1 in the original samples. The presented procedure was successfully applied for determination of lead content in real samples such as vegetables, waters, biological and soil samples with satisfactory results.
Keywords:Solid phase extraction;Colorimetry;Azo dyes;Lead determination;Environmental analysis
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