The current study indicates that octyl-beta-D-glucopyranoside (OGP) as a detergent which has the ability to make the lipid layer stiff. OGP was subjected for toxicity studies and in vitro cytotoxicty assays on cancerous HeLa and non-cancerous myoblasts H9c2 cell lines. Test against aquatic organisms were carried out in Artemia salina and LC50 values were calculated. Hemolytic activity tested for blood bio-compalibity showed hemolysis rate of 10-16%, followed by thrombolytic activity to burst the clots in blood. Also, the samples showed good lysis when compared to the standard streptokinase. Furthermore, alpha-amylase activity has been carried out to check the inhibition of alpha-amylase by the OGP. Finally, antibacterial activity has been tested against four different pathogens and their MIC values have been calculated.