In actual industrial processes, in addition to separation of just water and oil, separation of multiphase liquids and separation of organic solvents are also required. Here, we demonstrate successful multiphase liquid separation by simply applying different coating materials to aluminum meshes with a hierarchical microcubic and nanohole structure. A gravity-driven multiphase separation system was designed using these meshes as the separation media, and it showed high collection rate (99%) and high content ratio (95%). The fabricated separation system could sequentially separate liquids with surface tension of < 21.6, 21.6-32.0, and > 32.0 mN/m by choosing a filter with the proper surface energy. Given the small differences in the surface tension of the liquids used in this study, the separation system with a surface-energy-controlled filter can be an impressive tool to separate numerous liquid mixtures.